HHMI and Division of Biology, Caltech, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Mech Dev. 2002 Dec;119 Suppl 1:S203-9. doi: 10.1016/s0925-4773(03)00117-5.
The analysis of cell fate patterning during the vulval development of Caenorhabditis elegans has relied mostly on the direct observation of cell divisions and cell movements (cell lineage analysis). However, reconstruction of the developing vulva from EM serial sections has suggested seven different cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), many of which cannot be distinguished based on such observations. Here we report the vulval expression of seven genes, egl-17, cdh-3, ceh-2, zmp-1, B0034.1, T04B2.6 and F47B8.6 based on gfp, cfp and yfp (green fluorescent protein and color variants) reporter fusions. Each gene expresses in a specific subset of vulval cells, and is therefore useful as a marker for vulval cell fates. Together, expressions of markers distinguish six cell types, and reveal a strict temporal control of gene expression in the developing vulva.
秀丽隐杆线虫的生殖道发育过程中的细胞命运模式分析主要依赖于对细胞分裂和细胞运动的直接观察（细胞谱系分析）。然而，从 EM 连续切片重建发育中的生殖道表明存在七种不同的细胞类型（vulA、vulB1、vulB2、vulC、vulD、vulE 和 vulF），其中许多类型不能仅基于这些观察来区分。在这里，我们报告了七个基因的生殖道表达，这些基因基于 gfp、cfp 和 yfp（绿色荧光蛋白和颜色变体）报告融合，包括 egl-17、cdh-3、ceh-2、zmp-1、B0034.1、T04B2.6 和 F47B8.6。每个基因都在生殖道的特定细胞亚群中表达，因此可用作生殖道细胞命运的标记物。这些标记物的表达可以区分六种细胞类型，并揭示了发育中的生殖道中基因表达的严格时间控制。
