• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis.一种关键细胞周期激酶中的亚效突变导致生长迟缓和精子发生受损。
EMBO J. 2003 Oct 1;22(19):5260-72. doi: 10.1093/emboj/cdg497.
2
Cdc7 kinase complex: a key regulator in the initiation of DNA replication.细胞周期蛋白依赖性激酶7激酶复合物：DNA复制起始的关键调节因子。
J Cell Physiol. 2002 Mar;190(3):287-96. doi: 10.1002/jcp.10070.
3
CDC7 kinase phosphorylates serine residues adjacent to acidic amino acids in the minichromosome maintenance 2 protein.细胞周期蛋白依赖性激酶7（CDC7）使微小染色体维持蛋白2中酸性氨基酸附近的丝氨酸残基发生磷酸化。
Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11521-6. doi: 10.1073/pnas.0604990103. Epub 2006 Jul 24.
4
A Cdc7 kinase inhibitor restricts initiation of DNA replication and has antitumor activity.一种Cdc7激酶抑制剂可限制DNA复制的起始并具有抗肿瘤活性。
Nat Chem Biol. 2008 Jun;4(6):357-65. doi: 10.1038/nchembio.90. Epub 2008 May 11.
5
Genetic dissection of mammalian Cdc7 kinase: cell cycle and developmental roles.
Cell Cycle. 2004 Mar;3(3):300-4. Epub 2004 Mar 1.
6
High levels of Cdc7 and Dbf4 proteins can arrest cell-cycle progression.高水平的Cdc7和Dbf4蛋白可阻止细胞周期进程。
Eur J Cell Biol. 2005 Dec;84(12):927-38. doi: 10.1016/j.ejcb.2005.09.016. Epub 2005 Oct 26.
7
Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma.细胞周期蛋白依赖性激酶7（Cdc7）是上皮性卵巢癌生存的预测指标及新型治疗靶点。
Clin Cancer Res. 2009 Apr 1;15(7):2417-25. doi: 10.1158/1078-0432.CCR-08-1276. Epub 2009 Mar 24.
8
Cdc7 inhibition reveals a p53-dependent replication checkpoint that is defective in cancer cells.Cdc7抑制揭示了一种p53依赖的复制检查点，该检查点在癌细胞中存在缺陷。
Cancer Res. 2004 Oct 1;64(19):7110-6. doi: 10.1158/0008-5472.CAN-04-1547.
9
Drf1, a novel regulatory subunit for human Cdc7 kinase.Drf1，一种新型的人类Cdc7激酶调节亚基。
EMBO J. 2002 Jun 17;21(12):3171-81. doi: 10.1093/emboj/cdf290.
10
The Schizosaccharomyces pombe Cdc7 protein kinase required for septum formation is a client protein of Cdc37.裂殖酵母中隔膜形成所需的Cdc7蛋白激酶是Cdc37的一种客户蛋白。
Eukaryot Cell. 2007 Jul;6(7):1089-96. doi: 10.1128/EC.00080-07. Epub 2007 May 11.

引用本文的文献

1
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.Cdc7-Dbf4 激酶依赖性定位和磷酸化 MCM2-7 双六聚体的结构基础。
Nat Commun. 2022 May 25;13(1):2915. doi: 10.1038/s41467-022-30576-1.
2
Transcriptome analysis reveals potential mechanisms underlying differential heart development in fast- and slow-growing broilers under heat stress.转录组分析揭示了热应激下快、慢生长型肉鸡心脏发育差异的潜在机制。
BMC Genomics. 2017 Apr 13;18(1):295. doi: 10.1186/s12864-017-3675-9.
3
Genome-wide association study identifies 22 new loci for body dimension and body weight traits in a White Duroc×Erhualian F intercross population.全基因组关联研究在大白杜洛克×二花脸F2杂交群体中鉴定出22个与体型和体重性状相关的新基因座。
Asian-Australas J Anim Sci. 2017 Aug;30(8):1066-1073. doi: 10.5713/ajas.16.0679. Epub 2017 Jan 13.
4
Self-organization of meiotic recombination initiation: general principles and molecular pathways.减数分裂重组起始的自组织：一般原则和分子途径。
Annu Rev Genet. 2014;48:187-214. doi: 10.1146/annurev-genet-120213-092304.
5
Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast.Cdc7-Dbf4 是酵母减数分裂转录的基因特异性调节因子。
Mol Cell Biol. 2012 Jan;32(2):541-57. doi: 10.1128/MCB.06032-11. Epub 2011 Nov 21.
6
Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.多种途径可以绕过裂殖酵母 Hsk1 激酶在 DNA 复制起始中的必需作用。
J Cell Biol. 2011 Oct 31;195(3):387-401. doi: 10.1083/jcb.201107025. Epub 2011 Oct 24.
7
Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination.Cdc7 依赖的 Mer2 磷酸化促进酵母减数分裂重组的起始。
Genes Dev. 2008 Feb 1;22(3):398-410. doi: 10.1101/gad.1626608.
8
Hsk1 kinase is required for induction of meiotic dsDNA breaks without involving checkpoint kinases in fission yeast.在裂殖酵母中，Hsk1激酶是诱导减数分裂双链DNA断裂所必需的，且不涉及检查点激酶。
Proc Natl Acad Sci U S A. 2006 May 23;103(21):8131-6. doi: 10.1073/pnas.0602498103. Epub 2006 May 12.

本文引用的文献

1
Unique chromatin remodeling and transcriptional regulation in spermatogenesis.精子发生过程中独特的染色质重塑与转录调控。
Science. 2002 Jun 21;296(5576):2176-8. doi: 10.1126/science.1070963.
2
DNA replication in eukaryotic cells.真核细胞中的DNA复制。
Annu Rev Biochem. 2002;71:333-74. doi: 10.1146/annurev.biochem.71.110601.135425. Epub 2001 Nov 9.
3
Inactivation of Cdc7 kinase in mouse ES cells results in S-phase arrest and p53-dependent cell death.小鼠胚胎干细胞中Cdc7激酶的失活会导致S期停滞和p53依赖性细胞死亡。
EMBO J. 2002 May 1;21(9):2168-79. doi: 10.1093/emboj/21.9.2168.
4
Impaired neural tube closure, axial skeleton malformations, and tracheal ring disruption in TRAF4-deficient mice.TRAF4基因缺陷小鼠的神经管闭合受损、轴向骨骼畸形和气管环破坏。
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5585-90. doi: 10.1073/pnas.052124799. Epub 2002 Apr 9.
5
Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity.编码Cre重组酶的自切除逆转录病毒载体可克服Cre介导的细胞毒性。
Mol Cell. 2001 Jul;8(1):233-43. doi: 10.1016/s1097-2765(01)00295-7.
6
Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells.Cre重组酶在哺乳动物细胞中诱导的生长抑制和DNA损伤。
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9209-14. doi: 10.1073/pnas.161269798.
7
Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.裂殖酵母中Hsk1激酶对S期起始、复制检查点信号传导以及S期有丝分裂染色体结构维持的调控。
Mol Biol Cell. 2001 May;12(5):1257-74. doi: 10.1091/mbc.12.5.1257.
8
Analysis of C-MYC function in normal cells via conditional gene-targeted mutation.通过条件性基因靶向突变分析C-MYC在正常细胞中的功能。
Immunity. 2001 Jan;14(1):45-55. doi: 10.1016/s1074-7613(01)00088-7.
9
Involvement of the D-type cyclins in germ cell proliferation and differentiation in the mouse.D型细胞周期蛋白在小鼠生殖细胞增殖和分化中的作用。
Biol Reprod. 2000 Dec;63(6):1893-8. doi: 10.1095/biolreprod63.6.1893.
10
Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids.转基因小鼠精子细胞中非法的Cre依赖性染色体重排。
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13702-7. doi: 10.1073/pnas.240471297.

一种关键细胞周期激酶中的亚效突变导致生长迟缓和精子发生受损。

Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis.

作者信息

Kim Jung Min, Takemoto Naofumi, Arai Ken-ichi, Masai Hisao

机构信息

Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan.

出版信息

EMBO J. 2003 Oct 1;22(19):5260-72. doi: 10.1093/emboj/cdg497.

DOI:10.1093/emboj/cdg497
PMID:14517263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204480/
Abstract

Cdc7 kinase is essential for initiation of DNA replication. Cdc7(-/-) mouse embryonic stem (ES) cells are non-viable but their growth can be rescued by an ectopically expressed transgene (Cdc7(-/-)tg). Here we report that, despite the normal growth capability of Cdc7(-/-)tg ES cells, the mice with the identical genetic background exhibit growth retardation. Concomi tantly, Cdc7(-/-)tg embryonic fibroblasts (MEFs) display delayed S phase entry and slow S phase progression. Furthermore, spermatogenesis of Cdc7(-/-)tg mice is disrupted prior to pachytene stage of meiotic prophase I. The impairment in spermatogenesis correlates with the extremely low level of Cdc7 protein in testes, and is rescued by introducing an additional allele of transgene, which results in increase of Cdc7 expression. The increased level of Cdc7 also recovers the growth of Cdc7(-/-)tg MEFs and mice, indicating that the developmental abnormalities of Cdc7(-/-)tg mice are due to insufficiency of Cdc7 protein. Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals.

摘要

细胞分裂周期蛋白7（Cdc7）激酶对于DNA复制的起始至关重要。Cdc7基因敲除（Cdc7(-/-)）的小鼠胚胎干细胞无法存活，但其生长可通过异位表达的转基因（Cdc7(-/-)tg）得以挽救。在此我们报告，尽管Cdc7(-/-)tg胚胎干细胞具有正常的生长能力，但具有相同遗传背景的小鼠却表现出生长迟缓。与此同时，Cdc7(-/-)tg胚胎成纤维细胞（MEFs）进入S期延迟且S期进程缓慢。此外，Cdc7(-/-)tg小鼠的精子发生在减数分裂前期I的粗线期之前就受到破坏。精子发生的受损与睾丸中Cdc7蛋白的极低水平相关，通过引入额外的转基因等位基因可使其得到挽救，这导致Cdc7表达增加。Cdc7水平的升高也恢复了Cdc7(-/-)tg MEFs和小鼠的生长，表明Cdc7(-/-)tg小鼠的发育异常是由于Cdc7蛋白不足所致。我们的结果表明小鼠发育需要细胞周期调节因子达到临界水平，并提供了遗传学证据，证明Cdc7在哺乳动物减数分裂过程中起重要作用。