Hubácek J, Kratochvíl F, Polcrová A, Vojacek K
Allerg Immunol (Leipz). 1977;23(2):79-83.
Using a direct immunofluorescent staining method a qualitative determination of different immunoglobulin fractions in the tissue of hypertropic pharyngeal tonsils of children and youth suffering from repeated infections was performed. It was found that the immunofluorescent staining of IgG was stronger than of IgA. A difference with regard to the frequency of appearance between IgG and IgA could not be seen. On the other hand IgM staining was less frequent and strong. Extracellular IgG was present in germinal centres of the secondary lymphoid follicles, intracellular one was found in the immunocompetent cells. Extra- and intracellular IgG was more often demonstrable in the spaces of the lymphoid tissue, between the surface epithelial cells or around the glands and the blood cappilaries. The results seen here are in correlation with the values of the earlier performed quantitative estimations of the different immunoglobulin classes.
采用直接免疫荧光染色法，对反复感染的儿童和青少年增生性咽扁桃体组织中的不同免疫球蛋白组分进行了定性测定。结果发现，IgG的免疫荧光染色强于IgA。未观察到IgG和IgA在出现频率上的差异。另一方面，IgM染色较少见且强度较弱。细胞外IgG存在于次级淋巴滤泡的生发中心，细胞内IgG存在于免疫活性细胞中。细胞外和细胞内IgG在淋巴组织间隙、表面上皮细胞之间或腺体及毛细血管周围更常被检测到。此处观察到的结果与早期对不同免疫球蛋白类别的定量估计值相关。
