Lamelin J P, Thomasset N, Andre C, Brochier J, Revillard J P
Immunology. 1978 Sep;35(3):463-9.
Lymphoid cells from human palatine tonsils were identified on tissue sections by membrane or intracellular immunofluorescence (IF) staining. Used in an indirect technique, an anti-IgM and an anti-HTLA (human T lymphocyte antigen) antiserum gave complementary patterns of membrane IF, characteristic of the follicular organization. When serial sections were stained for each of the five classes, immunoglobulin-containing cells from all classes were found. Their relative frequencies were, in decreasing order: IgG: 61.6%; IgA: 17.3%; IgM: 12%; IgE: 7.5%; IgD: 1.6%. These differed from those of the gut-associated lymphoid tissue (IgA greater than IgG greater than IgM) and of the peripheral lymph nodes (IgG greater than IgM greater than IgA), but were close to those of mesenteric nodes. The absence of secretory component in tonsil was an additional difference from gut-associated lymphoid tissue and the relatively high proportion of IgE-containing cells, in the absence of recognized atopy, is another feature in common with mesenteric lymph nodes. Finally, slight differences between these results and those obtained on tonsil cell suspensions suggest that some degree of selection probably occurs during the isolation procedure.
通过膜或细胞内免疫荧光（IF）染色在组织切片上鉴定人腭扁桃体的淋巴细胞。在间接技术中，抗IgM和抗HTLA（人T淋巴细胞抗原）抗血清呈现出互补的膜IF模式，这是滤泡组织的特征。当对连续切片进行五类染色时，发现了所有类别的含免疫球蛋白细胞。它们的相对频率从高到低依次为：IgG：61.6%；IgA：17.3%；IgM：12%；IgE：7.5%；IgD：1.6%。这些与肠道相关淋巴组织（IgA＞IgG＞IgM）和外周淋巴结（IgG＞IgM＞IgA）不同，但与肠系膜淋巴结相近。扁桃体中缺乏分泌成分是与肠道相关淋巴组织的另一个差异，并且在没有公认的特应性情况下，含IgE细胞的相对高比例是与肠系膜淋巴结的另一个共同特征。最后，这些结果与扁桃体细胞悬液的结果之间的细微差异表明，在分离过程中可能发生了某种程度的选择。
