Martin T A, Harding K G, Jiang W G
Wound Healing Research Unit, University of Wales College of Medicine, Cardiff, UK.
Angiogenesis. 1999;3(1):69-76. doi: 10.1023/a:1009004212357.
This study sought to examine the effect of matrix-bound fibroblasts on angiogenesis and endothelial cell motility. Promotion of angiogenesis by matrix-bound fibroblasts was assessed using rat aortic ring and endothelial tube formation assays. Enhancement of human endothelial cell motility by matrix-bound fibroblasts was assessed using cytodex-2 bead and colloidal gold phagokinetic motility assays. Antibody to hepatocyte growth factor/scatter factor (HGF/SF) but not vascular endothelial growth factor (VEGf) decreased fibroblast-enhanced motility of endothelial cells. The promotion of tube formation by matrix-bound fibroblasts was neutralised with antibodies to HGF/SF and VEGf, both known promoters of angiogenesis. HGF/SF presence was detected by ELISA; whilst the presence of VEGf was detected by Western blotting. These data show that matrix-embedded fibroblasts regulate the motility of vascular endothelial cells and enhance angiogenesis, an effect partly attributed to production of angiogenesis-promoting cytokines HGF/SF and/or VEGf.
本研究旨在检测基质结合成纤维细胞对血管生成及内皮细胞运动性的影响。使用大鼠主动脉环和内皮管形成试验评估基质结合成纤维细胞对血管生成的促进作用。使用Cytodex-2微珠和胶体金吞噬动力学运动试验评估基质结合成纤维细胞对人内皮细胞运动性的增强作用。抗肝细胞生长因子/分散因子（HGF/SF）抗体而非血管内皮生长因子（VEGF）可降低成纤维细胞增强的内皮细胞运动性。基质结合成纤维细胞对管形成的促进作用被抗HGF/SF和VEGF抗体中和，这两种抗体均为已知的血管生成促进剂。通过ELISA检测HGF/SF的存在；同时通过蛋白质印迹法检测VEGF的存在。这些数据表明，基质包埋的成纤维细胞调节血管内皮细胞的运动性并增强血管生成，这一作用部分归因于促血管生成细胞因子HGF/SF和/或VEGF的产生。
