Yamazaki K, Nakazawa T, Matsunaga M, Kumazawa A, Kaneko T, Wakabayashi T
Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.
Jikken Dobutsu. 1992 Oct;41(4):523-7. doi: 10.1538/expanim1978.41.4_523.
We investigated motor function and pain sensation in the gracile axonal dystrophy (GAD) mutant mouse, using the tail-flick test and the rotarod test. GAD (gad/gad) and normal sib mice (gad/+ or +/+) were used between 5 and 11 weeks of age, during which time the behavioral signs of GAD mice shifted from sensory ataxia (about 4 to 8 weeks of age) to paresis (after about 9 weeks of age). In the tail-flick test, significant shortening of latency was observed at 6 and 8 weeks of age in female GAD mice, in comparison with normal female mice. This may be related to dysfunction or degeneration of axons in the fasiculus gracilis, whose collaterals are thought to control the transmission of nociceptive information. In the rotarod test, a cumulative chi 2 test showed significant reduction in the performance times of GAD mice beginning at 5 and 6 weeks of age in males and females, respectively, indicating that the rotarod test can detect the development of motor incoordination as early as these ages. The performance times of GAD mice dropped sharply from 9 weeks of age onwards, and this is believed to reflect the progression of paresis. The rotarod test therefore appears to be a good method of quantifying behavioral changes in GAD mice and to be applicable both to objective selection of GAD mice before 8 weeks of age and to evaluation of drugs to treat ataxia or paresis.
我们使用甩尾试验和转棒试验，对 gracile 轴索性营养不良（GAD）突变小鼠的运动功能和痛觉进行了研究。选用 5 至 11 周龄的 GAD（gad/gad）小鼠及其正常同窝小鼠（gad/+ 或 +/+），在此期间，GAD 小鼠的行为体征从感觉性共济失调（约 4 至 8 周龄）转变为轻瘫（约 9 周龄后）。在甩尾试验中，与正常雌性小鼠相比，雌性 GAD 小鼠在 6 周龄和 8 周龄时出现潜伏期显著缩短。这可能与薄束中轴突的功能障碍或退化有关，其侧支被认为控制伤害性信息的传递。在转棒试验中，累积卡方检验显示，雄性和雌性 GAD 小鼠分别在 5 周龄和 6 周龄时，其运动时间显著减少，这表明转棒试验能够早在这些年龄就检测到运动不协调的发展。GAD 小鼠的运动时间从 9 周龄起急剧下降，这被认为反映了轻瘫的进展。因此，转棒试验似乎是一种量化 GAD 小鼠行为变化的良好方法，既适用于 8 周龄前对 GAD 小鼠的客观筛选，也适用于对治疗共济失调或轻瘫药物的评估。
