Nakagata N, Matsumoto K, Anzai M, Takahashi A, Takahashi Y, Matsuzaki Y, Miyata K
NT Science Co., Ltd., Tokyo, Japan.
Jikken Dobutsu. 1992 Oct;41(4):537-40. doi: 10.1538/expanim1978.41.4_537.
Spermatozoa of a homozygous transgenic mouse, in which the firefly luciferase gene was expressed under the control of beta-actin promoter, were frozen at -196 degrees C. One fourth of the frozen sperm was later thawed and used for in vitro fertilization. Thirty-six of 65 oocytes (55.4%) developed to the 2-cell stage. All the 2-cell embryos were transferred to the oviducts of pseudopregnant recipients and 23 young (63.9%, 23/36) were born. All of young analyzed carried the transgene and showed the luciferase gene expression.
在β-肌动蛋白启动子控制下表达萤火虫荧光素酶基因的纯合转基因小鼠的精子，于-196℃冷冻。之后解冻了四分之一的冷冻精子用于体外受精。65个卵母细胞中有36个（55.4%）发育到2细胞期。所有2细胞胚胎被移植到假孕受体的输卵管中，出生了23只幼崽（63.9%，23/36）。所有分析的幼崽都携带转基因并显示出荧光素酶基因表达。
