Nakagata N
Nippon Institute for Biological Science, Tokyo, Japan.
J Reprod Fertil. 1993 Sep;99(1):77-80. doi: 10.1530/jrf.0.0990077.
Unfertilized oocytes (C57BL/6N and C3H/He) and epididymal spermatozoa (DBA/2N and Jcl:ICR) from mice were frozen separately and stored at -196 degrees C. After thawing, in vitro fertilization was performed using C57BL/6N oocytes and DBA/2N spermatozoa, C57BL/6N oocytes and Jcl:ICR spermatozoa, C3H/He oocytes and DBA/2N spermatozoa, and C3H/He oocytes and Jcl:ICR spermatozoa. Embryos developed to the two-cell stage by incubation in vitro were transferred to the oviducts of female recipients on the first day of pseudopregnancy (day when vaginal plug was confirmed). The rate of development to two-cell embryos in each group was in the 22-45% range. When these two-cell embryos were all transferred to recipients, offspring were produced from 23-35% of the embryos.
将小鼠的未受精卵母细胞（C57BL/6N和C3H/He）和附睾精子（DBA/2N和Jcl:ICR）分别冷冻并储存在-196℃。解冻后，使用C57BL/6N卵母细胞和DBA/2N精子、C57BL/6N卵母细胞和Jcl:ICR精子、C3H/He卵母细胞和DBA/2N精子以及C3H/He卵母细胞和Jcl:ICR精子进行体外受精。体外培养发育至二细胞期的胚胎在假孕第一天（确认有阴道栓的那天）被移植到雌性受体的输卵管中。每组发育至二细胞胚胎的比率在22%-45%范围内。当将这些二细胞胚胎全部移植到受体中时，23%-35%的胚胎产生了后代。
