采用激光诱导荧光检测的毛细管电泳法直接免疫测定雌酮。

Direct immunoassay of estrone by capillary electrophoresis with laser-induced fluorescence detection.

作者信息

Su Ping, Zhang Xin-Xiang, Chang Wen-Bao

机构信息

Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, China.

出版信息

Electrophoresis. 2003 Sep;24(18):3197-201. doi: 10.1002/elps.200305599.

DOI:10.1002/elps.200305599

PMID:14518044

Abstract

An immunoassay for estrone (E(1)) in women's serum, based on the competitive reaction between fluorescein-labeled complete antigen and E(1) with limited amount of anti-estrone monoclonal antibody is described. A thermally reversible hydrogel, poly-N-isopropylacrylamide (pNIPA), was added to the buffer to improve the reproducibility. With a laser-induced fluorescence (LIF) detector, the capillary electrophoretic immunoassay (CEIA) can be applied to determine E(1) at a concentration lower than 19.6 pg/mL. The E(1) levels in ten normal women's serum were measured at the range of 118.6-222.0 pg/mL.

摘要

描述了一种基于荧光素标记的完全抗原与雌酮（E(1)）在有限量抗雌酮单克隆抗体存在下的竞争反应，用于检测女性血清中雌酮（E(1)）的免疫分析方法。向缓冲液中添加了一种热可逆水凝胶，聚N-异丙基丙烯酰胺（pNIPA），以提高重现性。使用激光诱导荧光（LIF）检测器，毛细管电泳免疫分析（CEIA）可用于测定浓度低于19.6 pg/mL的E(1)。测量了十名正常女性血清中E(1)的水平，范围为118.6 - 222.0 pg/mL。