Zhou Jieyu, Xu Xueshu, Wang Yuxiao
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 283, Shanghai 200237, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 1;848(2):226-31. doi: 10.1016/j.jchromb.2006.10.027. Epub 2006 Nov 13.
A competitive immunoassay for detecting clenbuterol in urine was established by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). The clenbuterol was conjugated with bovine serum albumin (BSA), and then the derivative was labeled with fluorescein isothiocyanate (FITC) and competes for antibody with free clenbuterol in the sample. Under the optimal conditions, Free and bound FITC labeled clenbuterol was separated within 8 min with the relative standard deviation (R.S.D.) 0.72% for migration time and 2.8% for peak area. The detection limit reached 0.7 ng/ml.
建立了一种基于毛细管电泳（CE）结合激光诱导荧光（LIF）的竞争性免疫分析法，用于检测尿液中的克伦特罗。将克伦特罗与牛血清白蛋白（BSA）偶联，然后用异硫氰酸荧光素（FITC）标记该衍生物，并与样品中的游离克伦特罗竞争抗体。在最佳条件下，游离和结合FITC标记的克伦特罗在8分钟内分离，迁移时间的相对标准偏差（R.S.D.）为0.72%，峰面积的相对标准偏差为2.8%。检测限达到0.7 ng/ml。
