Liedke M, Karnbach C, Kalinin V, Herbst B, Frilling A, Broelsch C E
Chirurgische Klinik, Abteilung für Allgemeinchirurgie, Universitäts Krankenhaus Eppendorf, Hamburg.
Langenbecks Arch Chir Suppl Kongressbd. 1998;115(Suppl I):255-9.
The Gastro-Entero-Pancreatic System (GEP) defined a group of highly differentiated neuroendocrine tumors (Insulinomas, Gastrinomas, PP-omas, Somatostatinomas, Carcinoids). In this kind of tumors the multistage carcinogenesis is really unknown. Activated ras oncogenes could play an important role in initiation or process of carcinogenesis in different human cancers. The purpose of our study was to determe if H-ras or K-ras play a role in the carcinogenesis of GEP tumors. 20 paraffin embedded tumor tissues (1 Vipom, 1 Somatostatinom, 10 Carcinoids, 1 Gastrinoma, 4 livermetastasis of a Carcinoid, 3 Insulinomas) were analysed immunohistologic of H-ras and K-ras oncogene protein expression. Immunohistologic investigations showed a H-ras expression in 65% (13/20). K-ras protein could detected in 10% (2/20). On the basis of these data H-ras oncogene expression could play a role in the multistage carcinogenesis of GEP tumors.
胃肠胰系统（GEP）定义了一组高度分化的神经内分泌肿瘤（胰岛素瘤、胃泌素瘤、胰多肽瘤、生长抑素瘤、类癌）。在这类肿瘤中，多阶段致癌作用尚不清楚。激活的ras癌基因可能在不同人类癌症的致癌起始或过程中起重要作用。我们研究的目的是确定H-ras或K-ras是否在GEP肿瘤的致癌作用中发挥作用。对20个石蜡包埋的肿瘤组织（1个血管活性肠肽瘤、1个生长抑素瘤、10个类癌、1个胃泌素瘤、4个类癌肝转移灶、3个胰岛素瘤）进行了H-ras和K-ras癌基因蛋白表达的免疫组织学分析。免疫组织学研究显示，H-ras表达率为65%（13/20）。K-ras蛋白在10%（2/20）中可检测到。基于这些数据，H-ras癌基因表达可能在GEP肿瘤的多阶段致癌作用中发挥作用。
