Mancini N, Canducci F, Carletti S, Berardinelli E, Serafini G, Grieco A, Perotti M, Malcangi G, Danieli M G, Varaldo P E, Clementi M, Burioni R
Institute of Microbiology, University of Ancona, Ancona, Italy.
J Biol Regul Homeost Agents. 2003 Apr-Jun;17(2):183-7.
A complete understanding of the molecular features of humoral immune response could be of pivotal importance in the management of persistent viruses as HCV. In this study, 24 HCV-positive samples, characterized by classical virological parameters, are evaluated using a new assay for the quantitation of antibody subpopulations directed against discrete epitopes on surface glycoprotein E2, a key viral protein. The results, besides confirming the usefulness of this new approach, highlight the extreme heterogeneity of anti-HCV/E2 response as far as single epitopes are concerned. The specific epitopes under study are also demonstrated to be widely shared among different genotypes.
全面了解体液免疫反应的分子特征对于管理如丙型肝炎病毒（HCV）这样的持续性病毒可能至关重要。在本研究中，使用一种新的检测方法对24份以经典病毒学参数为特征的HCV阳性样本进行评估，该方法用于定量针对关键病毒蛋白表面糖蛋白E2上离散表位的抗体亚群。结果除了证实这种新方法的实用性外，还突出了就单个表位而言抗HCV/E2反应的极端异质性。所研究的特定表位还被证明在不同基因型之间广泛共享。
