Thieme Karena, Schnell Ingo
Max-Planck-Institut für Polymerforschung, and Institut für Organische Chemie, Universität Mainz, Germany.
J Am Chem Soc. 2003 Oct 8;125(40):12100-1. doi: 10.1021/ja037374n.
By introducing dipolar recoupling methods to high-resolution magic-angle spinning (HRMAS) NMR spectroscopy, a class of experiments has been delevoped that allows the measurement of residual dipole-dipole couplings of approximately 1 Hz in weakly immobilized molecules. Using homonuclear 1H-1H recoupling, distances of up to approximately 8 A can be selectively determined, while heteronuclear 1H-13C recoupling provides access to dynamic order parameters of individual molecular segments on the order of approximately 10-3. The experiments are demonstrated on functionalized oligopeptides that are attached to polymer resins.
通过将偶极耦合方法引入高分辨率魔角旋转（HRMAS）核磁共振光谱学，已开发出一类实验，可用于测量弱固定化分子中约1 Hz的残余偶极-偶极耦合。使用同核1H-1H耦合，可选择性地确定高达约8 Å的距离，而异核1H-13C耦合则可获取单个分子片段的动态序参数，其量级约为10^-3。这些实验在连接到聚合物树脂上的功能化寡肽上得到了验证。
