Orden J A, Yuste M, Cid D, Piacesi T, Martínez S, Ruiz-Santa-Quiteria J A, De la Fuente R
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense 28040, Madrid, Spain.
Vet Microbiol. 2003 Oct 17;96(2):203-15. doi: 10.1016/s0378-1135(03)00238-4.
The types of the eae and espB genes of 178 attaching and effacing Escherichia coli (AEEC) strains isolated from diarrhoeic and healthy ruminants were investigated by PCR. Six types of the eae gene: beta (beta), gamma1 (gamma-1), gamma2 (gamma-2), epsilon (epsilon), zeta (zeta) and iota (iota), and three types of the espB gene: alpha, beta and gamma were identified in the strains studied. Moreover, three strains were negative to all the types of the eae gene tested. The types beta and gamma2 in healthy cattle, beta, gamma2 and epsilon in healthy sheep and goats, and beta in diarrhoeic calves, lambs and goat kids were the most frequent types of the eae gene among the strains studied. Although the eaebeta gene was the most prevalent among AEEC from healthy and diarrhoeic ruminants, the percentages of AEEC strains with this type found in this study in diarrhoeic animals (66.7-100%) were higher than those found in healthy animals (33.3-40.6%). Thus, these data suggest that AEEC strains with the eaebeta gene are associated with neonatal diarrhoea in ruminants. The eaegamma1, eaezeta and eaeiota genes were found in low percentages in the strains studied (4.5, 2.8 and 7.3%, respectively). All the types of the eae gene, except the type iota, showed a close correlation with the types of the espB gene: the eaebeta and eae epsilon genes with the espBbeta gene, the eaegamma2 and eaezeta genes with the espBalpha gene and the eaegamma1 gene with the espBgamma gene.
采用聚合酶链反应（PCR）对从腹泻和健康反刍动物中分离出的178株紧密黏附性大肠杆菌（AEEC）的eae和espB基因类型进行了研究。在所研究的菌株中鉴定出6种eae基因类型：β型、γ1型、γ2型、ε型、ζ型和ι型，以及3种espB基因类型：α型、β型和γ型。此外，有3株菌株对所有检测的eae基因类型均呈阴性。在所研究的菌株中，健康牛中的β型和γ2型、健康绵羊和山羊中的β型、γ2型和ε型，以及腹泻犊牛、羔羊和山羊羔中的β型是最常见的eae基因类型。尽管eaeβ基因在来自健康和腹泻反刍动物的AEEC中最为普遍，但本研究中在腹泻动物中发现的具有该类型的AEEC菌株百分比（66.7 - 100%）高于在健康动物中发现的百分比（33.3 - 40.6%）。因此，这些数据表明具有eaeβ基因的AEEC菌株与反刍动物的新生腹泻有关。在所研究的菌株中，eaeγ1、eaeζ和eaeι基因的比例较低（分别为4.5%、2.8%和7.3%）。除ι型外，所有eae基因类型均与espB基因类型密切相关：eaeβ和eaeε基因与espBβ基因、eaeγ2和eaeζ基因与espBα基因、eaeγ1基因与espBγ基因。
