反刍动物黏附与脱屑大肠杆菌肠上皮细胞脱屑位点基因的聚合酶链反应分型
Polymerase chain reaction typing of genes of the locus of enterocyte effacement of ruminant attaching and effacing Escherichia coil.
作者信息
Yuste María, Orden José A, De La Fuente Ricardo, Ruiz-Santa-Quiteria José A, Cid Dolores, Martínez-Pulgarín Susana, Domínguez-Bernal Gustavo
机构信息
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Can J Vet Res. 2008 Oct;72(5):444-8.
Abstract
The variability of the tir, espA, and espD genes of the locus of enterocyte effacement (LEE) in 185 attaching and effacing Escherichia coli (AEEC) strains isolated from healthy and diarrheic cattle, sheep, and goats was investigated by polymerase chain reaction. Nineteen of the strains were enterohemorrhagic E. coli (EHEC); the other 166 were enteropathogenic E. coli (EPEC). The combinations of the tir and esp genes were associated with the variants of the eae gene but not with a strain's belonging to the EPEC or EHEC group, animal species, or health status (healthy or diarrheic) of the animal. In addition, most of the strains showed the same combinations of LEE genes and serogroups as have been found in AEEC strains isolated from humans, which indicates that ruminants seem to be an EPEC reservoir for humans.
摘要
通过聚合酶链反应研究了从健康和腹泻的牛、羊及山羊中分离出的185株黏附和损伤性大肠杆菌（AEEC）菌株中肠上皮细胞损伤位点（LEE）的tir、espA和espD基因的变异性。其中19株为肠出血性大肠杆菌（EHEC）；其他166株为肠致病性大肠杆菌（EPEC）。tir和esp基因的组合与eae基因的变体相关，但与菌株属于EPEC或EHEC组、动物种类或动物的健康状况（健康或腹泻）无关。此外，大多数菌株显示出与从人类分离出的AEEC菌株相同的LEE基因和血清群组合，这表明反刍动物似乎是人类的EPEC储存宿主。
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