Stahl J, Wobus A M, Ihrig S, Lutsch G, Bielka H
Max Delbrück Center of Molecular Medicine, Berlin-Buch, Federal Republic of Germany.
Differentiation. 1992 Sep;51(1):33-7. doi: 10.1111/j.1432-0436.1992.tb00677.x.
Murine embryonal carcinoma and embryonic stem cell lines were investigated with regard to the occurrence of the small heat shock protein hsp25 during cell growth and differentiation. In the embryonal carcinoma cell line F9 considerable constitutive levels of hsp25 were observed which could be slightly increased by treatment with retinoic acid. No hsp25 was found, however, in the embryonal carcinoma cell line PCC4. When analyzing the pluripotent embryonal carcinoma cell line P19 and the pluripotent embryonic stem cell line BLC6, both characterized by high differentiation capacity, no hsp25 was observed under cell culture conditions maintaining the undifferentiated state. Induction of differentiation caused by prolonged cell culture, retinoic acid treatment, or embryoid body formation, however, resulted in an increase of the level of hsp25. The finding that hsp25 is accumulated in a differentiation-dependent manner suggests that this protein is associated with processes involved in differentiation. Therefore, hsp25 can be regarded as a marker of differentiation in the investigated embryonal carcinoma cell line P19 and the embryonic stem cell line BLC6.
对小鼠胚胎癌细胞系和胚胎干细胞系在细胞生长和分化过程中小分子热休克蛋白hsp25的出现情况进行了研究。在胚胎癌细胞系F9中，观察到hsp25有相当高的组成性水平，用视黄酸处理后可略有增加。然而，在胚胎癌细胞系PCC4中未发现hsp25。当分析具有高分化能力的多能胚胎癌细胞系P19和多能胚胎干细胞系BLC6时，在维持未分化状态的细胞培养条件下未观察到hsp25。然而，长时间细胞培养、视黄酸处理或胚状体形成所诱导的分化导致hsp25水平升高。hsp25以依赖分化的方式积累这一发现表明，该蛋白与分化过程有关。因此，hsp25可被视为所研究的胚胎癌细胞系P19和胚胎干细胞系BLC6中分化的标志物。
