Bunaprasert Tanom, Hadlock Tessa, Marler Jennifer, Kobler James, Cowan Douglas, Faquin William, Varvares Mark
Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114, USA.
Laryngoscope. 2003 Oct;113(10):1792-7. doi: 10.1097/00005537-200310000-00025.
OBJECTIVES/HYPOTHESIS: Because current tongue reconstructive methods introduce adynamic, variably sensate tissue into the mouth, the critical functions of the tongue in articulation and deglutition may be compromised. The objective of this work was to introduce a combination of myoblasts and scaffolding material into rat hemiglossectomy defects and to examine the extent of neomuscle formation in the reconstructed area, under the hypothesis that the presence of myoblasts leads to formation of new muscle.
Randomized, prospective animal study.
Myoblasts were harvested from neonatal Lewis rats, and a growth factor enriched collagen gel was prepared. Syngeneic adult animals received either hemiglossectomy alone or reconstruction with one of four experimental reconstructive preparations: collagen gel alone, collagen gel with suspended myoblasts, the gel-cell combination in undifferentiated muscle construct form by way of tissue culture for 7 days in a preformed mold, or differentiated constructs, cultured in myoblast fusion medium. After 6 or 16 weeks, animal weight gain was recorded, animals were killed, and the tongues harvested. The tissue was examined histologically, and quality of the muscular regenerate was rated on a scale according to predefined criteria.
Animals in all groups gained weight appropriately. In groups receiving hemiglossectomy alone or acellular (gel only) reconstruction, there was significant scarring and lack of neomuscle formation. In groups receiving myoblast transplantation, either by way of gel suspension or in the form of undifferentiated or differentiated constructs, muscle quality was superior to controls.
Myoblast transplantation into hemiglossectomy defects appears to lead to new muscle formation and does not inhibit normal weight gain in animals after tongue implantation.
目的/假设：由于目前的舌重建方法会将无活力、感觉功能各异的组织引入口腔，舌在发音和吞咽中的关键功能可能会受到损害。本研究的目的是将成肌细胞与支架材料组合植入大鼠半舌切除缺损处，研究重建区域新肌肉形成的程度，假设成肌细胞的存在会导致新肌肉的形成。
随机、前瞻性动物研究。
从新生Lewis大鼠中获取成肌细胞，并制备富含生长因子的胶原凝胶。同基因成年动物接受单纯半舌切除术或四种实验性重建制剂之一进行重建：单纯胶原凝胶、含悬浮成肌细胞的胶原凝胶、通过在预制模具中组织培养7天形成的未分化肌肉构建体形式的凝胶 - 细胞组合，或在成肌细胞融合培养基中培养的分化构建体。6周或16周后，记录动物体重增加情况，处死动物并摘取舌头。对组织进行组织学检查，并根据预定义标准对肌肉再生质量进行评分。
所有组的动物体重均适当增加。在接受单纯半舌切除术或无细胞（仅凝胶）重建的组中，有明显瘢痕形成且无新肌肉形成。在接受成肌细胞移植的组中，无论是通过凝胶悬浮液还是未分化或分化构建体的形式，肌肉质量均优于对照组。
将成肌细胞移植到半舌切除缺损处似乎会导致新肌肉形成，且不抑制动物在舌植入后的正常体重增加。
