Huang Guilin, Li Longjiang, Wen Yuming
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2003 Dec;21(6):432-4.
The purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.
Using purified, subcultured myoblast of neonatal rats and type I collagen gels as extracellular matrix (ECM) and scaffold, tissue engineered muscle was transplanted in the face of syngeneic nutured rats.
Tissue engineered myoblast generated and differentiated in vitro were observed with microscope. Myoblast fused each other and formed myofibers in the face of rats, nerve fibers and vessels regeneration could also be found in some samples. Postoperative electromiographs showed the myofibers were active when stimulating the nerve trunk that innervates the engineered facial muscle.
Tissue engineered method is hopeful to be used as a new technique to reconstruct defects of facial muscle.
本文旨在探讨利用组织工程化成肌细胞修复SD大鼠面部肌肉缺损。
采用新生大鼠纯化、传代培养的成肌细胞，以Ⅰ型胶原凝胶作为细胞外基质（ECM）和支架，将组织工程化肌肉移植到同基因饲养大鼠面部。
显微镜下观察到体外培养的组织工程化成肌细胞生长并分化。大鼠面部的成肌细胞相互融合形成肌纤维，部分标本中还可见神经纤维和血管再生。术后肌电图显示，刺激支配工程化面部肌肉的神经干时，肌纤维有活性。
组织工程方法有望成为修复面部肌肉缺损的新技术。
