Horiuchi Y, Agulnik A, Figueroa F, Tichy H, Klein J
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
Genet Res. 1992 Aug;60(1):43-52. doi: 10.1017/s0016672300030652.
Three anonymous chromosome 17 DNA markers, D17Tu36, D17Tu43, and D17Le66B, differentiate between house mouse species and/or between t chromosomes. The D17Tu36 probe, which maps near the Fu locus and to the In(17)4 on t chromosomes, identifies at least 15 haplotypes, each haplotype characterized by a particular combination of DNA fragments obtained after digestion with the Taq I restriction endonuclease. Ten of these haplotypes occur in Mus domesticus, while the remaining five occur in M. musculus. In each of these two species, one haplotype is borne by t chromosomes while the other haplotypes are present on non-t chromosomes. The D17Tu43 probe, which maps near the D17Leh122 locus and to the In(17)3 on t chromosomes, also identifies at least 15 haplotypes in Taq I DNA digests, of which nine occur in M. domesticus and six in M. musculus. One of the nine M. domesticus haplotypes is borne by t chromosomes, the other haplotypes are borne by non-t chromosomes; two of the six M. musculus haplotypes are borne by t chromosomes and the remaining four by non-t chromosomes. Some of the D17Tu43 haplotypes are widely distributed in a given species, while others appear to be population-specific. Exceptions to species-specificity are found only in a few mice captured near the M. domesticus-M. musculus hybrid zone or in t chromosomes that appear to be of hybrid origin. The D17Leh66B probe, which maps to the In(17)2, distinguishes three haplotypes of M. domesticus-derived t chromosomes and one haplotype of M. musculus-derived t chromosomes. Because of these characteristics, the three markers are well suited for the study of mouse population genetics in general and of t chromosome population genetics in particular. A preliminary survey of wild M. domesticus and M. musculus populations has not uncovered any evidence of widespread introgression of genes from one species to the other; possible minor introgressions were found only in the vicinity of the hybrid zone. Typing of inbred strains has revealed the contribution of only M. domesticus DNA to the chromosome 17 of the laboratory mouse.
三个匿名的17号染色体DNA标记，即D17Tu36、D17Tu43和D17Le66B，可区分小家鼠物种和/或t染色体。D17Tu36探针定位于Fu位点附近以及t染色体上的In(17)4，可识别至少15种单倍型，每种单倍型的特征是经Taq I限制性内切酶消化后获得的特定DNA片段组合。其中10种单倍型出现在小家鼠中，其余5种出现在小鼠中。在这两个物种中，每种都有1种单倍型由t染色体携带，而其他单倍型存在于非t染色体上。D17Tu43探针定位于D17Leh122位点附近以及t染色体上的In(17)3，在Taq I DNA消化产物中也识别出至少15种单倍型，其中9种出现在小家鼠中，6种出现在小鼠中。小家鼠的9种单倍型中有1种由t染色体携带，其他单倍型由非t染色体携带；小鼠的6种单倍型中有2种由t染色体携带，其余4种由非t染色体携带。一些D17Tu43单倍型在特定物种中广泛分布，而其他单倍型似乎具有种群特异性。仅在小家鼠 - 小鼠杂交区域附近捕获的少数小鼠或似乎是杂交起源的t染色体中发现了物种特异性的例外情况。定位于In(17)2的D17Leh66B探针可区分3种小家鼠来源的t染色体单倍型和1种小鼠来源的t染色体单倍型。由于这些特性，这三个标记非常适合一般小鼠群体遗传学研究，特别是t染色体群体遗传学研究。对野生小家鼠和小鼠群体的初步调查未发现任何一个物种的基因广泛渗入另一个物种的证据；仅在杂交区域附近发现了可能的少量基因渗入。近交系的分型显示，实验室小鼠17号染色体仅含有小家鼠的DNA。
