Mizuno K, Vincek V, Figueroa F, Klein J
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Federal Republic of Germany.
Immunogenetics. 1989;30(2):105-11. doi: 10.1007/BF02421538.
The mouse x Chinese hamster cell line R4 4-1 contains only one mouse chromosome, the bulk of which corresponds to Mus musculus chromosomes 17 and 18 (MMU17 and MMU18, respectively). A genomic library was prepared from the R4 4-1 DNA, and a mouse clone was isolated from the library, which-with the help of somatic cell hybrids-could be mapped to the MMU17. A locus defined by a 2.7-kb long Bam HI probe from this clone was designated D17Tu5 (Tu for Tübingen). The locus proved to be polymorphic among inbred strains and wild mice. By testing of recombinant inbred strains and partial t haplotypes, the D17Tu5 locus could be mapped to a position between the D17Leh66E and D17Rp17 loci within the t complex. Two alleles were found at this locus, D17Tu5a and D17Tu5b, defined by Taq I restriction fragment length polymorphism. Both alleles are present among inbred strains and wild mice of the species M. domesticus. All complete t haplotypes tested carry the D17Tu5a allele and all tested wild mice of the species M. musculus, with the exception of those bearing t haplotypes, carry the D17Tu5b allele. Additional alleles are found in some populations of wild mice and in other species of the genus Mus. The distribution of the two alleles among the inbred strains correlates well with their known or postulated genealogy. Their distribution between the two species of Mus and among the mice with T haplotypes suggests a relatively recent origin of the t haplotypes.
小鼠×中国仓鼠细胞系R4 4-1仅包含一条小鼠染色体，其大部分对应于小家鼠染色体17和18（分别为MMU17和MMU18）。从R4 4-1 DNA制备了一个基因组文库，并从该文库中分离出一个小鼠克隆，借助体细胞杂种可将其定位到MMU17上。来自该克隆的一个2.7 kb长的Bam HI探针所定义的基因座被命名为D17Tu5（Tu代表图宾根）。该基因座在近交系和野生小鼠中被证明是多态性的。通过对重组近交系和部分t单倍型的检测，D17Tu5基因座可定位到t复合体中D17Leh66E和D17Rp17基因座之间的位置。在该基因座发现了两个等位基因，D17Tu5a和D17Tu5b，由Taq I限制性片段长度多态性定义。这两个等位基因都存在于家鼠属的近交系和野生小鼠中。所有检测的完整t单倍型都携带D17Tu5a等位基因，所有检测的小家鼠野生小鼠（携带t单倍型的除外）都携带D17Tu5b等位基因。在一些野生小鼠群体和小家鼠属的其他物种中发现了额外的等位基因。这两个等位基因在近交系中的分布与它们已知的或推测的谱系密切相关。它们在小家鼠的两个物种之间以及带有T单倍型的小鼠中的分布表明t单倍型的起源相对较近。
