Wang X W, Hynes M J, Davis M A
Molecular and Evolutionary Systematics Group, Research School of Biological Sciences, Australian National University, Canberra.
Gene. 1992 Dec 1;122(1):147-54. doi: 10.1016/0378-1119(92)90042-n.
We have isolated the Aspergillus oryzae homologue of the amdR regulatory gene of Aspergillus nidulans by cross hybridization. Sequence analysis and functional studies have shown that the amdR genes are highly conserved and functionally interchangeable between the two species. The homology between the two genes extends throughout most of the coding sequences, including sequences encoding the DNA-binding domain and putative activation domains. Two regions of nonconserved sequence were also identified. Studies using various amdS::lacZ fusion constructs indicate that the A. oryzae gene product binds similar sequences and responds to inducer in a similar manner to the A. nidulans protein. Inactivation of the A. oryzae gene results in the inability to grow on gamma-amino-butyric acid (GABA) as a carbon and/or nitrogen source indicating that GABA utilization is amdR-dependent in A. oryzae as it is in A. nidulans.
我们通过交叉杂交分离出了米曲霉中构巢曲霉amdR调控基因的同源物。序列分析和功能研究表明，amdR基因在这两个物种之间高度保守且功能可互换。这两个基因之间的同源性延伸至大部分编码序列，包括编码DNA结合结构域和推定激活结构域的序列。还鉴定出了两个非保守序列区域。使用各种amdS::lacZ融合构建体进行的研究表明，米曲霉基因产物结合的序列与构巢曲霉蛋白相似，并且对诱导剂的反应方式也相似。米曲霉基因的失活导致其无法以γ-氨基丁酸（GABA）作为碳源和/或氮源生长，这表明米曲霉中GABA的利用与构巢曲霉一样依赖于amdR。
