Hynes M J
Department of Genetics, University of Melbourne, Parkville, Victoria, Australia.
Antonie Van Leeuwenhoek. 1994;65(3):179-82. doi: 10.1007/BF00871944.
The amdS gene codes for an acetamidase enzyme that hydrolyses acetamide to acetate and ammonium thus providing A. nidulans with a source of carbon and nitrogen. The exceptionally favourable genetics of this system combined with molecular analysis have enabled many regulatory circuits affecting amdS to be identified genetically. Characterization of the regulatory genes and the definition of the cis-acting sites involved have been done using both in vivo and in vitro mutagenesis. Recent results on the analysis of the system are presented.
amdS基因编码一种酰胺酶，该酶将乙酰胺水解为乙酸盐和铵，从而为构巢曲霉提供碳源和氮源。该系统特别有利的遗传学特性与分子分析相结合，使得许多影响amdS的调控回路能够通过遗传学方法得以鉴定。利用体内和体外诱变技术对调控基因进行了表征，并确定了相关的顺式作用位点。本文介绍了该系统分析的最新结果。
