Frostegård J, Wramsby M L, Wu R, Bremme K
Department of Rheumatology, Karolinska Hospital Stockholm, Sweden.
Am J Obstet Gynecol. 2003 Aug;189(2):568-73. doi: 10.1067/s0002-9378(03)00550-7.
The purpose of this study was to determine the antibody levels against platelet-activating factor (PAF), PAF-like lipids lysophosphatidylcholine (LPC), cardiolipin (CL), and oxidized low-density lipoprotein (LDL) in recurrent abortion.
Eigthy-five women with a history of at least 3 recurrent spontaneous abortions (55 women with primary abortion [31.4+/-5.1 years old]; 30 women with secondary abortion [33+/-4.5 years old]) were compared with 48 age-matched control subjects (33+/-4.4 years old) who had delivered without complications. Antibodies were measured using enzyme-linked immunosorbent assay (ELISA).
Antibodies against PAF and antibodies against oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (oxPAPC) were raised significantly in the group with primary abortion, as compared to control subjects (P<.05) and not significantly higher than in secondary abortion; antibodies against oxidized LDL, LPC, or CL levels did not differ between groups.
Antibodies against platelet-activating factor and antibodies against platelet-activating factor-like lipid oxPAPC are associated with primary recurrent abortion and may function as novel disease markers. Whether these antibodies also are pathogenic (eg, by interacting with endothelial cells in placenta or by interfering with platelet-activating factor during embryonal development) remains to be shown.
本研究旨在测定复发性流产患者体内抗血小板活化因子（PAF）、类PAF脂质溶血磷脂酰胆碱（LPC）、心磷脂（CL）和氧化型低密度脂蛋白（LDL）的抗体水平。
将85例有至少3次复发性自然流产史的女性（55例原发性流产女性[31.4±5.1岁]；30例继发性流产女性[33±4.5岁]）与48例年龄匹配的无并发症分娩的对照受试者（33±4.4岁）进行比较。采用酶联免疫吸附测定（ELISA）法检测抗体。
与对照受试者相比，原发性流产组中抗PAF抗体和抗氧化型1-棕榈酰-2-花生四烯酰-sn-甘油-3-磷酸胆碱（oxPAPC）抗体显著升高（P<0.05），且不比继发性流产组显著更高；各组间抗氧化型LDL、LPC或CL水平无差异。
抗血小板活化因子抗体和抗类血小板活化因子脂质oxPAPC抗体与原发性复发性流产相关，可能作为新的疾病标志物。这些抗体是否也具有致病性（例如，通过与胎盘内皮细胞相互作用或在胚胎发育过程中干扰血小板活化因子）仍有待证实。
