Knerr Ina, Weigel Corina, Linnemann Knud, Dötsch Jörg, Meissner Udo, Fusch Christoph, Rascher Wolfgang
Department of Pediatrics, University of Erlangen-Nuremberg, Erlangen, Germany.
Am J Obstet Gynecol. 2003 Aug;189(2):583-8. doi: 10.1067/s0002-9378(03)00538-6.
The purpose of this study was to test the hypothesis that hypoxia down-regulates placental syncytin, which could play a role in altered placentogenesis; we investigated the influence of hypoxia on syncytin and its receptor ASCT2 gene expression in BeWo cells and in ex vivo perfused human cotyledons.
BeWo cells were incubated with deferoxamine or cobalt chloride under normoxia and hypoxia. Additionally, a model of dually ex vivo perfused cotyledons was applied. Under hypoxic and cobalt chloride stimuli syncytin, ASCT2, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, and beta(2)-microglobulin (beta(2)-MG) messenger RNAs were analyzed with the use of real-time polymerase chain reaction.
Hypoxia, deferoxamine, and cobalt chloride markedly decreased syncytin messenger RNA in BeWo cells, whereas ASCT2 messenger RNA was not altered significantly. In isolated perfused cotyledons, hypoxia also reduced syncytin (P<.05) but not ASCT2 messenger RNA.
Our data provide first evidence that syncytin gene expression is down-regulated by hypoxia, which strengthens the hypothesis that syncytin is reduced in disturbed pregnancies in the course of placental hypoxia.
本研究旨在验证以下假说，即缺氧会下调胎盘融合素，而这可能在胎盘形成改变中发挥作用；我们研究了缺氧对BeWo细胞及体外灌注人胎盘小叶中融合素及其受体ASCT2基因表达的影响。
将BeWo细胞在常氧和缺氧条件下与去铁胺或氯化钴孵育。此外，应用双体外灌注胎盘小叶模型。在缺氧和氯化钴刺激下，使用实时聚合酶链反应分析融合素、ASCT2、甘油醛-3-磷酸脱氢酶（GAPDH）、β-肌动蛋白和β2-微球蛋白（β2-MG）的信使核糖核酸。
缺氧、去铁胺和氯化钴显著降低了BeWo细胞中融合素信使核糖核酸的水平，而ASCT2信使核糖核酸水平无明显改变。在分离的灌注胎盘小叶中，缺氧也降低了融合素水平（P<0.05），但未降低ASCT2信使核糖核酸水平。
我们的数据首次证明缺氧会下调融合素基因表达，这强化了以下假说，即在胎盘缺氧过程中，融合素在妊娠异常时会减少。
