Andersen H L, Duch B U, Nielsen J B, Joergensen B, Ledet T
Institute of Experimental Clinical Research, University of Aarhus, Skejby Hospital, 8200 Aarhus N, Denmark.
Urol Res. 2003 Dec;31(6):363-7. doi: 10.1007/s00240-003-0333-2. Epub 2003 Oct 1.
In this study, an animal model was developed for the examination of urethral strictures (US). Through a resectoscope, a resection was made in the urethras of 15 male rabbits. After 30 days, the rabbits were evaluated with urethrography, impedance planimetry and either histology or the determination of collagen content. Fifteen rabbits serving as controls were evaluated in the same way. Three rabbits in the resection group and one in the control group died before evaluation. Urethrography demonstrated a stricture in the remaining 12 animals in the resection group. The urethras of the control animals were all normal. Impedance planimetry confirmed that the luminal cross sectional area (CSA) of the strictures was significantly smaller than the CSA of the corresponding part of the urethra in the control group. No difference in CSA was found 1 cm proximal to the stricture. The strictures consisted of densely woven collagen which sent tongues into the adjacent normal parts of the urethra. No difference in collagen content was found between the two groups either at the stricture site or 1 cm proximally. The described method of producing US in the rabbit model was very consistent with all operated animals developing a stricture. The model might prove valuable in evaluating new methods for the treatment of US.
在本研究中，建立了一种用于检查尿道狭窄（US）的动物模型。通过电切镜，在15只雄性兔的尿道进行切除。30天后，对兔进行尿道造影、阻抗平面测量以及组织学检查或胶原蛋白含量测定。以同样方式对15只作为对照的兔进行评估。切除组中有3只兔和对照组中有1只兔在评估前死亡。尿道造影显示切除组中其余12只动物存在狭窄。对照动物的尿道均正常。阻抗平面测量证实，狭窄部位的管腔横截面积（CSA）明显小于对照组中尿道相应部位的CSA。在狭窄近端1 cm处未发现CSA有差异。狭窄由致密编织的胶原蛋白组成，其呈舌状延伸至尿道相邻的正常部位。在狭窄部位或近端1 cm处，两组之间的胶原蛋白含量均未发现差异。在兔模型中所描述的产生尿道狭窄的方法在所有接受手术的动物中均非常一致地导致了狭窄。该模型可能在评估治疗尿道狭窄的新方法方面具有价值。
