Amer Soheir M, Donya Souria M, Aly Fawzia A E
Department of Genetics and Cytology, National Research Center, Dokki, Cairo, Egypt.
Arch Toxicol. 2003 Dec;77(12):712-21. doi: 10.1007/s00204-003-0464-9. Epub 2003 Oct 1.
Swiss mice were fed for 2, 4 and 8 weeks wheat grains treated with 1, 2, and 4 g benomyl/kg and stored for 6 and 12 weeks. The maximum effect of benomyl on the induction of chromosomal aberrations was observed after feeding mice for 8 weeks with wheat grains treated with 4 g benomyl/kg and stored for 12 weeks. Its proportion differed significantly in bone marrow and spermatocyte cells, 15+/-0.51% vs. 13.4+/-0.66%, respectively, from that in nontreated mice (background level), 4.4+/-0.24% and 3.8+/-0.20%, respectively. Lengthening the storage period of treated wheat grains caused a dose-dependent increase in the frequency of sister chromatid exchanges: 8.61+/-0.34 vs. 4.16+/-0.06/cell. The proportion of sperm-head abnormalities increased by lengthening the period of storage and feeding: 7.7+/-0.41% vs. 3.25+/-0.12%. In another experiment mice were orally treated by gavage with benomyl at 50, 100, 150, 200 mg/kg; a significant and dose-dependent increase in sperm-head abnormalities was observed. These findings demonstrate that benomyl (a 50% wettable powder formulation) and its residues in wheat grains are genotoxic in mice.
给瑞士小鼠喂食经1、2和4克/千克苯菌灵处理并储存6周和12周的小麦颗粒，持续2周、4周和8周。在用4克/千克苯菌灵处理并储存12周的小麦颗粒喂养小鼠8周后，观察到苯菌灵对诱导染色体畸变的最大影响。其在骨髓细胞和精母细胞中的比例与未处理小鼠（背景水平）相比有显著差异，骨髓细胞中分别为15±0.51%，精母细胞中分别为13.4±0.66%，而未处理小鼠的背景水平分别为4.4±0.24%和3.8±0.20%。延长处理过的小麦颗粒的储存期会导致姐妹染色单体交换频率呈剂量依赖性增加：从4.16±0.06/细胞增加到8.61±0.34/细胞。精子头部异常的比例随着储存期和喂养期的延长而增加：从3.25±0.12%增加到7.7±0.41%。在另一项实验中，给小鼠经口灌胃50、100、150、200毫克/千克的苯菌灵；观察到精子头部异常有显著的剂量依赖性增加。这些发现表明，苯菌灵（50%可湿性粉剂配方）及其在小麦颗粒中的残留物对小鼠具有遗传毒性。
