Nentwich I, Pazdírková A, Koberská I, Pokojská E, Szépfalusi Z, Lokaj J
Institut der klinischen Immunologie und Allergologie, Masaryk-Universität in Brünn, Tschechien.
Klin Padiatr. 2003 Sep-Oct;215(5):275-9. doi: 10.1055/s-2003-42664.
Hypoallergenic infant formulas (HAF) were developed for atopy prevention in infants with high risk of atopy if these cannot be breastfed. HAF mount an antigen-specific immune response in infants. The aim of the study was to analyse the immune response in infants fed with a new infant formula based on a whey hydrolysate (HAF) and to compare it with that of exclusively breastfed controls.
Plasma concentrations of cow milk-specific IgE were analysed in 94 infants with high risk of atopy, 44 were exclusively breastfed, 50 were fed with HAF. In addition, cow milk-specific IgG antibodies (26 breastfed, 30 fed with HAF) as well as proliferation of periph-eral blood mononuclear cells to bovine beta-lactoglobulin (BLG) (41 breastfed, 47 fed with HAF) were tested. Specific IgE and IgG antibodies were determined using enzymoimmunometric assay (Alastat). Cellular proliferation was measured using tritiated thymidine incorporation assay after 6 day stimulation with BLG.
Elevated IgE to cow milk antibodies (> 0.35 kU/L) were detected in two infants from the breastfed group and in one from the HAF-fed group. The plasma concentrations of milk specific IgG antibodies in HAF-fed infants were insignificantly higher than those in breastfed ones. No significant difference was found in bovine BLG-specific cell proliferation between both groups.
Concerning the properties investigated like antigenicity, allergenicity and immunogenicity, the extensively hydrolysed whey based hypoallergenic formula does not significantly differ from mother milk in 6 month-old infants with an increased atopy risk.
低敏婴儿配方奶粉（HAF）是为无法进行母乳喂养的、有特应性疾病高风险的婴儿预防特应性疾病而研发的。HAF可在婴儿体内引发抗原特异性免疫反应。本研究旨在分析食用一种基于乳清水解物的新型婴儿配方奶粉（HAF）的婴儿的免疫反应，并将其与纯母乳喂养的对照组婴儿的免疫反应进行比较。
对94名有特应性疾病高风险的婴儿的牛奶特异性IgE血浆浓度进行了分析，其中44名婴儿纯母乳喂养，50名婴儿食用HAF。此外，还检测了牛奶特异性IgG抗体（26名母乳喂养，30名食用HAF）以及外周血单个核细胞对牛β-乳球蛋白（BLG）的增殖反应（41名母乳喂养，47名食用HAF）。使用酶免疫分析法（Alastat）测定特异性IgE和IgG抗体。在用BLG刺激6天后，使用氚标记胸腺嘧啶核苷掺入法测量细胞增殖。
在母乳喂养组的两名婴儿和HAF喂养组的一名婴儿中检测到牛奶抗体IgE升高（>0.35 kU/L）。食用HAF的婴儿的牛奶特异性IgG抗体血浆浓度略高于母乳喂养的婴儿。两组之间牛BLG特异性细胞增殖没有显著差异。
就所研究的抗原性、致敏性和免疫原性等特性而言，对于有特应性疾病风险增加的6个月大婴儿，这种广泛水解的基于乳清的低敏配方奶粉与母乳没有显著差异。
