Banerjee U C
Institute of Microbial Technology, Chandigarh, India.
Folia Microbiol (Praha). 1992;37(4):256-60. doi: 10.1007/BF02814559.
beta-Glucosidase from Curvularia lunata was immobilized in pellets of polyacrylamide, sodium alginate and agar. The activity of the enzyme was estimated at different times by measuring the absorbance of a solution into which 2-nitrophenol was released by the enzyme. The effect of pH and temperature was studied to select the optimum conditions. Thermostability of the beta-glucosidase in each of the carriers was assessed over a period of 12-26 d. The immobilized enzyme on all the three carriers retained its activity longer than free enzyme did. Polyacrylamide was the best carrier both in terms of thermostability and of reusability of the immobilized enzyme preparations. The Michaelis constant (Km) for each of the immobilized enzyme preparation was calculated.
来自新月弯孢霉的β-葡萄糖苷酶被固定在聚丙烯酰胺、海藻酸钠和琼脂颗粒中。通过测量酶释放出2-硝基苯酚的溶液的吸光度，在不同时间估计酶的活性。研究了pH和温度的影响以选择最佳条件。在12 - 26天的时间内评估了β-葡萄糖苷酶在每种载体中的热稳定性。固定在所有三种载体上的酶比游离酶保留其活性的时间更长。就固定化酶制剂的热稳定性和可重复使用性而言，聚丙烯酰胺是最佳载体。计算了每种固定化酶制剂的米氏常数（Km）。
