Banerjee U C, Vohra R M
Institute of Microbial Technology, Chandigarh, India.
Folia Microbiol (Praha). 1991;36(4):343-6. doi: 10.1007/BF02814506.
A Curvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30 degrees C were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found to be 5.2 and 50 degrees C, respectively. Under optimum conditions, the enzyme had a Km (guaiacol) of 0.75 mmol/L and a V of 1.50 CU min-1 ml-1. Some divalent metal ions inhibited laccase activity at very low concentrations.
从土壤中分离出的一种弯孢霉菌被发现对以愈创木酚为底物具有漆酶活性。该微生物在含有酵母提取物、蛋白胨和葡萄糖的培养基中产生胞外漆酶。发现初始培养基pH 4.0和培养温度30℃最适合酶的最大产量。漆酶活性的最适pH和温度分别为5.2和50℃。在最佳条件下，该酶的Km（愈创木酚）为0.75 mmol/L，V为1.50 CU min-1 ml-1。一些二价金属离子在极低浓度下就会抑制漆酶活性。
