[通过DNA杂交检测不同毒力钩端螺旋体菌株间的基因组DNA同源性]

[Assay of genomic DNA homology among strains of different virulent leptospira by DNA hybridization].

作者信息

Huang N, Dai B

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1992 Jun;23(2):122-5.

Abstract

Nick translation labelling technique was applied to the preparation of two 32P labelled genomic DNA probes from L. interrogans serovar Lai strain 017 and Leptonema illini strain 3055, respectively. Dot-blotting and Southern-blotting with DNA of five strains leptospira from different genus and species were performed. The results showed that certain differences among the L. interrogans, L. biflexa and Leptonema illini could be detected by endonuclease assay. L. interrogans strain 017 with L. biflexa strain Patoc I and Leptonema illini 3055 strain exhibited very little homology. L. interrogans strains 017, 601, and 245 from different serogroup and serovar had a high degree of homology. Leptonema illini strain 3055 showed little homology with L. interrogans and L. biflexa. Therefore DNA hybridization may be used as a tool for the identification and classification of leptospira.

摘要

尼克翻译标记技术分别应用于从问号钩端螺旋体赖型菌株017和伊利尼细螺旋体菌株3055制备两种32P标记的基因组DNA探针。对来自不同属和种的五种钩端螺旋体菌株的DNA进行了点杂交和Southern杂交。结果表明，通过核酸内切酶分析可以检测到问号钩端螺旋体、双曲钩端螺旋体和伊利尼细螺旋体之间存在某些差异。问号钩端螺旋体菌株017与双曲钩端螺旋体菌株帕托克I和伊利尼细螺旋体3055菌株表现出极少的同源性。来自不同血清群和血清型的问号钩端螺旋体菌株017、601和245具有高度同源性。伊利尼细螺旋体菌株3055与问号钩端螺旋体和双曲钩端螺旋体表现出极少的同源性。因此，DNA杂交可作为钩端螺旋体鉴定和分类的工具。