Zhang Y, Dai B
Hua Xi Yi Ke Da Xue Xue Bao. 1992 Jun;23(2):130-2.
Photobiotin- and 32P-labelled DNA probes of L. interrogans sv. lai strain 017 were produced and the DNA and leptospires were dotted on the NC filter and hybridized. The results showed that photobiotin and 32P-labelled DNA probes could detect DNAs of homology. The smallest amount of DNA that could be detected with the probes were 5pg and 1pg, and the smallest numbers of pathogenic leptospires were 5 x 10(3) and 10(3), respectively. Nonpathogenic leptospires. L. biflexa sv. patoc strain Patoc 1, L. illini strain 3055, Escherichia coli, Bacillus aerogenes capsulatus, and Salmonella anatis, could not be detected by the probes. The study demonstrates that leptospires DNA probe could be produced by labelling the DNA with photobiotin. It has higher sensitivity and specificity and can be used for detection of leptospires in the field and clinic.
制备了问号状钩端螺旋体赖型菌株017的光生物素和32P标记的DNA探针，并将DNA和钩端螺旋体点样于硝酸纤维素滤膜上进行杂交。结果表明，光生物素和32P标记的DNA探针能检测出同源DNA。探针能检测到的最小DNA量分别为5pg和1pg，能检测到的致病性钩端螺旋体的最小数量分别为5×10(3)和10(3)。非致病性钩端螺旋体，双曲钩端螺旋体帕托血清型菌株帕托1、伊利尼钩端螺旋体菌株3055、大肠杆菌、产气荚膜芽孢杆菌和鸭沙门氏菌，均不能被探针检测到。该研究表明，通过用光生物素标记DNA可制备钩端螺旋体DNA探针。它具有较高的敏感性和特异性，可用于现场和临床钩端螺旋体的检测。
