Sha Wei, Weng Xin-hua, Xiao He-ping, He Guo-jun
Department of Tuberculosis, Pneumology Hospital, Shanghai 200433, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Sep;26(9):544-7.
To observe the resistance of Mycobacterium abscessus to rifampin and to investigate if there is any mutation of rpoB gene in strains with high minimal inhibitory concentration (MIC).
Mycobacterium abscessus was identified with both biochemical methods and PCR-RFLP. The MICs of rifampin to all the clinical strains and the type strain ATCC19977 were determined. DNA sequences were obtained from a 1272 bp fragment of the rpoB gene from either low or high rifampin MIC strains using PCR amplification.
Only one strain had low MICs of 4 microg/ml. MICs of all the other 14 strains, as well as the type stain were above 128-256 microg/ml. Although there were some differences in nucleotide sequence of rpoB gene, all strains had the same amino acid sequence without any mutations.
Mycobacterium abscessus showed a high resistance to rifampin. Mutations of rpoB gene do not seem to be the responsible mechanism.
观察脓肿分枝杆菌对利福平的耐药性，并调查最低抑菌浓度（MIC）较高的菌株中rpoB基因是否存在突变。
采用生化方法和PCR-RFLP对脓肿分枝杆菌进行鉴定。测定利福平对所有临床菌株及标准菌株ATCC19977的MIC。使用PCR扩增从利福平MIC低或高的菌株的rpoB基因的1272 bp片段中获取DNA序列。
仅1株的MIC低至4μg/ml。其他14株菌株以及标准菌株的MIC均高于128 - 256μg/ml。尽管rpoB基因的核苷酸序列存在一些差异，但所有菌株的氨基酸序列相同，无任何突变。
脓肿分枝杆菌对利福平表现出高度耐药性。rpoB基因的突变似乎不是其耐药机制。
Zotero 插件