Gibellini Davide, Monari Paola, Sassi Monica, Re Maria Carla
Section of Microbiology, Department of Clinical and Experimental Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
J Clin Virol. 2003 Dec;28(3):341-3. doi: 10.1016/s1386-6532(03)00080-5.
A hemi-nested PCR approach was adopted to detect HTLV-1 infection in clinical samples of peripheral blood mononuclear cells (PBMCs) from subjects with positive or indeterminate serological results. Our results showed that the hemi-nested PCR quickly solved the diagnostic query, detecting the presence of proviral HTLV-1 DNA in two of the 252 patients with inconclusive serological results. The main advantage of this method are the typology of DNA extraction, allowing a consistent DNA recovery without amplification problems, the rapidity (4-5 hours), the performance of the assay and its comparable or better sensitivity than other HTLV-1 PCR formats.
采用半巢式聚合酶链反应（PCR）方法检测血清学结果呈阳性或不确定的受试者外周血单个核细胞（PBMC）临床样本中的人嗜T淋巴细胞病毒1型（HTLV-1）感染情况。我们的结果表明，半巢式PCR快速解决了诊断疑问，在252例血清学结果不明确的患者中，检测到2例存在HTLV-1前病毒DNA。该方法的主要优点包括DNA提取类型，可实现一致的DNA回收且无扩增问题、速度快（4-5小时）、检测性能以及与其他HTLV-1 PCR方法相比具有相当或更好的灵敏度。
