Gorinstein Shela, Yamamoto Kazutaka, Kobayashi Shoichi, Taniguchi Hajime, Pawelzik Elke, Delgado-Licon Efren, Shaoxian Yue, Hongliang Sun, Ayala Alma Leticia Martinez, Trakhtenberg Simon
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University-Hadassah Medical School, P.O.B. 12065, Jerusalem 91120, Israel.
Int J Food Sci Nutr. 2003 Nov;54(6):427-35. doi: 10.1080/09637480310001622305.
Amaranth, soybean and maize were screened for proteins and their nutritional value. Isopropanol-soluble protein and buffer-soluble protein fractions were extracted from seeds and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison of the identity and differences between investigated plants was carried out by the obtained SDS-PAGE electrophoretic patterns, and their microstructure was determined by scanning electron microscopy. Electrophoretic patterns of extracted proteins have shown that the main protein subunits were concentrated between 10 and 50 kDa. Variations were found in major fractions and minor bands as well as in the fine structure. The microstructure of pseudocereal and cereal protein fractions was inter-related with the results obtained by their electrophoretic separation. Pseudocereal amaranth can be used as a nutritive substitute of cereal maize in functional foods.
对苋菜、大豆和玉米进行了蛋白质及其营养价值的筛选。从种子中提取异丙醇可溶性蛋白和缓冲液可溶性蛋白组分，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分离。通过所得的SDS-PAGE电泳图谱对所研究植物之间的异同进行比较，并通过扫描电子显微镜确定其微观结构。提取蛋白的电泳图谱表明，主要蛋白质亚基集中在10至50 kDa之间。在主要组分和次要条带以及精细结构中均发现了差异。伪谷物和谷物蛋白组分的微观结构与通过电泳分离获得的结果相互关联。伪谷物苋菜可作为功能性食品中谷物玉米的营养替代品。
