Hamada Shigeki, Ishiyama Keiki, Sakulsingharoj Chotipa, Choi Sang-Bong, Wu Yujia, Wang Changlin, Singh Salvinder, Kawai Naoko, Messing Joachim, Okita Thomas W
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
Plant Cell. 2003 Oct;15(10):2265-72. doi: 10.1105/tpc.013821.
Prolamine and glutelin RNAs are localized to two subdomains of the cortical endoplasmic reticulum (ER), the protein body ER and the cisternal ER, in developing rice seeds. The addition of nearly full-length prolamine sequences at the 3' untranslated region of a reporter RNA redirects its localization from the cisternal ER to the protein body ER. Deletion analysis of prolamine RNA sequences indicates the presence of two partially redundant cis elements required for protein body ER targeting. The addition of glutelin 3' untranslated region to protein body ER cis sequences, however, redirects RNA localization to the cisternal ER. These results indicate that there are at least two regulated RNA transport pathways as well as a constitutive pathway to the cortical ER.
醇溶蛋白和谷蛋白RNA定位于发育中的水稻种子皮层内质网（ER）的两个亚结构域，即蛋白体ER和潴泡ER。在报告RNA的3'非翻译区添加几乎全长的醇溶蛋白序列，可将其定位从潴泡ER重定向至蛋白体ER。醇溶蛋白RNA序列的缺失分析表明，存在两个靶向蛋白体ER所需的部分冗余顺式元件。然而，将谷蛋白3'非翻译区添加到蛋白体ER顺式序列中，会将RNA定位重定向至潴泡ER。这些结果表明，至少存在两条受调控的RNA转运途径以及一条通向皮层内质网的组成型途径。
