Choi Sun-Uk, Lee Chang-Kwon, Hwang Yong-Il, Kinosita Hiroshi, Nihira Takuya
International Center for Biotechnology, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Arch Microbiol. 2003 Oct;180(4):303-7. doi: 10.1007/s00203-003-0591-y. Epub 2003 Sep 3.
The presence of gamma-butyrolactone autoregulators and their receptor proteins were investigated in five representative strains of non- Streptomyces actinomycetes producing commercially important secondary metabolites. Ethyl acetate extracts of culture were assayed using wild-type S. virginiae for virginiae butanolide, S. lavendulae FRI-5 for IM-2, and S. griseus HH1 for A-factor. Actinoplanes teichomyceticus and Amycolatopsis mediterranei were shown to produce autoregulators. Corresponding autoregulator-binding activities were found in the crude cell-free lysates of these strains, using the binding assay with tritium-labeled autoregulator analogues as ligands, which suggests that non- Streptomyces actinomycetes might have autoregulator-dependent signaling cascades.
在五株具有代表性的非链霉菌放线菌中研究了γ-丁内酯自调控因子及其受体蛋白的存在情况，这些放线菌可产生具有重要商业价值的次生代谢产物。使用弗吉尼亚链霉菌野生型菌株检测培养物的乙酸乙酯提取物中的弗吉尼亚丁醇内酯，用薰衣草链霉菌FRI-5检测IM-2，用灰色链霉菌HH1检测A因子。结果表明，游动放线菌和地中海拟无枝酸菌可产生自调控因子。利用以氚标记的自调控因子类似物作为配体的结合试验，在这些菌株粗制的无细胞裂解物中发现了相应的自调控因子结合活性，这表明非链霉菌放线菌可能具有依赖自调控因子的信号级联反应。
