Lewis Michael R, Jia Fang
Department of Veterinary Medicine and Surgery, University of Missouri-Columbia, Columbia, Missouri 65211, USA.
J Cell Biochem. 2003 Oct 15;90(3):464-72. doi: 10.1002/jcb.10641.
Labeled oligonucleotide analogues for antisense imaging of messenger RNA (mRNA) have great potential for detection of endogenous gene expression in vivo. Successful antisense imaging may be useful for detecting cellular gene expression patterns and early molecular changes in disease. Conclusive demonstration of this technique has been hindered by formidable challenges in surmounting biological barriers and detecting low concentrations of target mRNA. Recent advances in the development of novel antisense molecules, high specific activity radiolabeling chemistry, sophisticated drug targeting technology, and complementary molecular imaging modalities make it quite possible that true antisense imaging will be realized in the near future.
用于信使核糖核酸（mRNA）反义成像的标记寡核苷酸类似物在体内检测内源性基因表达方面具有巨大潜力。成功的反义成像可能有助于检测细胞基因表达模式和疾病中的早期分子变化。由于在克服生物屏障和检测低浓度靶mRNA方面面临巨大挑战，这项技术的确证一直受到阻碍。新型反义分子、高比活度放射性标记化学、先进的药物靶向技术以及互补分子成像模式的最新进展使得在不久的将来实现真正的反义成像成为可能。
