Paroo Zain, Corey David R
Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-9041, USA.
J Cell Biochem. 2003 Oct 15;90(3):437-42. doi: 10.1002/jcb.10626.
The development of methods for non-invasive, real-time imaging of gene expression would provide powerful tools for biomedical research and medical diagnostics. A broadly applicable strategy for achieving this goal is the use of complementary oligonucleotide probes for recognition of mRNA. The major challenge for molecular imaging is the development of specific and efficient transducers for signaling probe-target interaction. This review summarizes the strengths and limitations of reported molecular approaches for imaging of mRNA expression and discusses the challenges to development of in vivo methods.
用于基因表达的非侵入性实时成像方法的发展将为生物医学研究和医学诊断提供强大的工具。实现这一目标的一种广泛适用策略是使用互补寡核苷酸探针来识别信使核糖核酸（mRNA）。分子成像的主要挑战是开发用于信号传导探针 - 靶标相互作用的特异性和高效换能器。本综述总结了已报道的用于mRNA表达成像的分子方法的优缺点，并讨论了体内方法开发面临的挑战。
