Golyshevskaia V I, Irtuganova O A, Smirnova N S, Domotenko L V
Probl Tuberk Bolezn Legk. 2003(8):34-7.
The accelerated nitrate reductase method (NRM) developed at the Central Research Institute of Tuberculosis versus the automatic assay of drug sensitivity by means of a BACTEC 960 bacteriological analyzer was assessed. NRM was carried out, by using the Lówenstein-Jensen medium for 10 days. It is based on the detection of alive Mycobacteria tuberculosis, by recording their enzymatic activity. The study showed a good agreement of the results obtained by NRM with those obtained on a BACTEC 960 analyzer. Agreements were found for 52 isolates in 47 (90.4%) cases, the results disagreed in the testing of 5 (9.6%) cultures. The results of NRM were identical to those for 21 of the 22 cultures sensitive on a BACTEC 960 device; the coincidence was 95.5%. The sensitivity of NRM ranged from 88.2% (for rifampicin) to 96.3% (for isoniazid) and the specificity did from 96% (for isoniazid) to 100% (for streptomycin, rifampicin, and ethambutol). The positive prognostic value of NRM was 100% (for streptomycin, rifampicin, and ethambutol) and 96.3% (for isoniazid). The negative prognostic value of NRM ranged from 94.6 to 96.8% for individual drugs. The efficiency of NRM (a ratio of the number of correct results to the total number of results) was greater than 0.96, which suggests that this method and the BACTEC MGIT 960 AST technique may be regarded as rather comparable. The testing of NRM versus the automatic BACTEC MGIT 960 AST technique has indicated that the former may be successfully used to determine the sensitivity of Mycobacteria to the critical concentrations of first-line antituberculous agents.
对中央结核病研究所开发的加速硝酸盐还原酶法（NRM）与使用BACTEC 960细菌分析仪自动检测药物敏感性进行了评估。NRM采用罗-琴培养基进行，持续10天。它基于通过记录结核分枝杆菌的酶活性来检测存活的结核分枝杆菌。研究表明，NRM获得的结果与BACTEC 960分析仪获得的结果具有良好的一致性。在47例（90.4%）病例中的52株分离株中发现结果一致，在5例（9.6%）培养物的检测中结果不一致。NRM的结果与BACTEC 960设备上22株敏感培养物中的21株相同；符合率为95.5%。NRM的敏感性范围为88.2%（利福平）至96.3%（异烟肼），特异性范围为96%（异烟肼）至100%（链霉素、利福平和乙胺丁醇）。NRM的阳性预测值对链霉素、利福平和乙胺丁醇为100%，对异烟肼为96.3%。NRM对个别药物的阴性预测值范围为94.6%至96.8%。NRM的效率（正确结果数与结果总数的比率）大于0.96，这表明该方法与BACTEC MGIT 960 AST技术相当。将NRM与自动BACTEC MGIT 960 AST技术进行比较测试表明，前者可成功用于确定结核分枝杆菌对一线抗结核药物临界浓度的敏感性。
