Rowe Gerald E, Margaritis Argyrios, Wei Ning
Department of Chemical and Biochemical Engineering, Faculty of Engineering, The University of Western Ontario, London, Ontario, Canada N6A 5B9.
Biotechnol Prog. 2003 Sep-Oct;19(5):1439-43. doi: 10.1021/bp030018o.
The specific oxygen uptake rate (q(O)2, respiration rate) of Bacillus thuringiensis subsp. kurstaki HD-1 was very high at inoculation and was found to decrease essentially monotonically throughout both vegetative growth phase and transition phase under different batch culture conditions. Average q(O)2 values decreased from 8-10 mmol/g h at 1 h after inoculation to less than 2 mmol/g h by the time growth ended. The results are shown to be consistent with the few previous reports on q(O)2 in B. thuringiensis in the literature but also novel in that this pattern of monotonic decline has not been described previously. Both pH control and EDTA in low concentration shortened the vegetative growth phase and reduced the 10 h biomass concentration. Using plots of q(O)2 versus specific growth rate, mu, biomass yield based on the oxygen used for growth, was calculated for transition phase to be 0.041-0.047 g/mmol, consistent with literature values. The same plot also showed that the presence of EDTA resulted in an atypical q(O)2-mu trajectory and apparently much higher biomass yield from the oxygen consumed.
苏云金芽孢杆菌库尔斯塔克亚种HD-1的比氧摄取率（q(O)₂，呼吸速率）在接种时非常高，并且发现在不同的分批培养条件下，在整个营养生长阶段和过渡阶段基本单调下降。平均q(O)₂值从接种后1小时的8-10 mmol/g·h下降到生长结束时的低于2 mmol/g·h。结果表明与文献中关于苏云金芽孢杆菌q(O)₂的少数先前报道一致，但也有新颖之处，即这种单调下降模式以前没有被描述过。pH控制和低浓度的EDTA都缩短了营养生长阶段并降低了10小时时的生物量浓度。使用q(O)₂与比生长速率μ的关系图，计算出过渡阶段基于用于生长的氧气的生物量产率为0.041-0.047 g/mmol，与文献值一致。同样的图还表明，EDTA的存在导致了非典型的q(O)₂-μ轨迹，并且从消耗的氧气中获得的生物量产率明显更高。
