Shimada M, Akagi N, Goto H, Watanabe H, Nakanishi M, Hirose Y, Watanabe M
Department of Anatomy, Osaka Medical College, Japan.
J Anat. 1992 Feb;180 ( Pt 1)(Pt 1):89-95.
In order to study the factors responsible for glucose uptake in the mouse hippocampus, microvessel and astroglial cell densities were measured and compared in each laminal region. Microvessel density was examined on histologically prepared sections after injection of Indian ink and measured by means of an image analyser. Astroglial cell density was determined after the cells were stained immunohistochemically. Microvessel and astroglial cell densities were determined in 10 different hippocampal structures. Microvessel and astroglial cell densities were strongly correlated in all layers except the pyramidal cell layers. The highest density of perfused microvessels was found in the stratum lacunosum-moleculare, compared with other regions, and the lowest values were found in the stratum lucidum and dentate granular cell layer. Among pyramidal cell layers, microvessel density in sector CA3a was significantly higher than that in CA1.
为了研究小鼠海马体中葡萄糖摄取的相关因素，对每个分层区域的微血管密度和星形胶质细胞密度进行了测量和比较。在注射印度墨水后，对组织学制备的切片进行微血管密度检查，并通过图像分析仪进行测量。在细胞进行免疫组织化学染色后确定星形胶质细胞密度。在10个不同的海马结构中测定了微血管和星形胶质细胞密度。除锥体细胞层外，所有层的微血管和星形胶质细胞密度都高度相关。与其他区域相比，在分子层-腔隙层中发现灌注微血管的密度最高，而在透明层和齿状颗粒细胞层中发现最低值。在锥体细胞层中，CA3a区的微血管密度明显高于CA1区。
