Herold M L, Constanzer M L, Matuszewski B K
Merck Sharp and Dohme Research Laboratories, West Point, PA 19486.
J Chromatogr. 1992 Oct 23;581(2):227-36. doi: 10.1016/0378-4347(92)80276-v.
A sensitive (50 pg/ml) method for the determination of heptylphysostigmine in human plasma is described. The procedure is based on liquid-liquid extraction of the drug from buffered plasma, and analysis of the concentrated organic extract using high-performance liquid chromatography on a silica column, under normal-phase chromatographic conditions, with fluorescence detection. Physostigmine was used as an internal standard. The assay has been fully validated in the concentration range 50-2000 pg/ml and utilized for the analysis of clinical samples from subjects dosed with heptylphysostigmine.
本文描述了一种用于测定人血浆中庚基毒扁豆碱的灵敏方法（检测限为50 pg/ml）。该方法基于从缓冲血浆中液-液萃取药物，并使用硅胶柱在正相色谱条件下进行高效液相色谱分析浓缩后的有机萃取物，采用荧光检测。毒扁豆碱用作内标。该测定法在50-2000 pg/ml的浓度范围内已得到充分验证，并用于分析给予庚基毒扁豆碱的受试者的临床样本。
