Zecca L, Radice D, Mosca A, Pagella P G
Istituto di Tecnologie Biomediche Avanzate CNR, Milan, Italy.
J Chromatogr. 1993 May 19;615(1):169-73. doi: 10.1016/0378-4347(93)80305-n.
Heptylphysostigmine is a new and very promising cholinergic drug for the treatment of Alzheimer disease. A method has been developed for its determination in plasma with a detection limit of 50 pg/ml. The drug was extracted in n-hexane by a simple one-step procedure, after buffering with sodium bicarbonate. Samples were analysed on a 25 cm x 4.6 mm I.D. silica column (5 microns particle size) using a mixture of acetonitrile, methanol and ammonium nitrate as mobile phase. Since this molecule is quite unstable in plasma, pyridostigmine bromide was added to samples to limit the decomposition. Physostigmine was employed as internal standard. The molecule was electrochemically detected by oxidizing potential (+0.75 V). The method was applied to the analysis of blood samples taken from one healthy volunteer administered this drug. In the same subject the inhibition rate of acetylcholinesterase in plasma and red cells was also measured.
庚基毒扁豆碱是一种新型且非常有前景的用于治疗阿尔茨海默病的胆碱能药物。已开发出一种在血浆中测定它的方法，检测限为50 pg/ml。在用碳酸氢钠缓冲后，通过简单的一步程序将该药物用正己烷萃取。样品在一根25 cm×4.6 mm内径的硅胶柱（粒径5微米）上进行分析，使用乙腈、甲醇和硝酸铵的混合物作为流动相。由于该分子在血浆中相当不稳定，向样品中加入溴吡斯的明以限制分解。毒扁豆碱用作内标。通过氧化电位（+0.75 V）对该分子进行电化学检测。该方法应用于对一名服用此药物的健康志愿者采集的血样进行分析。在同一受试者中，还测定了血浆和红细胞中乙酰胆碱酯酶的抑制率。
