小鼠端粒酶催化亚基mTERT基因在SD大鼠睾丸中的表达及其意义。

Expression of mouse telomerase catalytic subunit mTERT gene in testis of SD rats and its significance.

作者信息

Ye Zhewei, Chen Xiaochun, Yang Shuhua, Chen Jiang, Xiong Yali, Lu Gongcheng

机构信息

Department of Orthopedics, Xiehe hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):288-90. doi: 10.1007/BF02829517.

DOI:10.1007/BF02829517

PMID:14526437

Abstract

To study the expression of mTERT gene in the testis of SD rats and its significance, in situ hybridization (ISH) techniques were used to detect the expression of telomerase gene mTERT mRNA in the testis of SD rats. The expression of mTERT was detectable in different-age male SD rats testis. There was a positive correlation between the expression of mTERT and the location of germ cells (spermatogonia, spermatocyte, spermatid). In Sertoli cells, leydig cell and spermatozoa, telomerase mTERT was not detected. Type A spermatogonia expressed the highest level of telomerase mTERT mRNA. Our results suggest that the expression of mTERT gene in the testis of SD rats is of lifetime and coincide with the telomerase activity.

摘要

为研究mTERT基因在SD大鼠睾丸中的表达及其意义，采用原位杂交（ISH）技术检测端粒酶基因mTERT mRNA在SD大鼠睾丸中的表达。在不同年龄雄性SD大鼠睾丸中均可检测到mTERT的表达。mTERT的表达与生殖细胞（精原细胞、精母细胞、精子细胞）的位置呈正相关。在支持细胞、间质细胞和精子中未检测到端粒酶mTERT。A型精原细胞表达的端粒酶mTERT mRNA水平最高。我们的结果表明，mTERT基因在SD大鼠睾丸中的表达具有终生性，且与端粒酶活性一致。