一种哺乳动物端粒酶相关蛋白。

A mammalian telomerase-associated protein.

作者信息

Harrington L, McPhail T, Mar V, Zhou W, Oulton R, Bass M B, Arruda I, Robinson M O

机构信息

Arruda, Ontario Cancer Institute-Amgen Institute, Department of Medical Biophysics, University of Toronto, 620 University Avenue, Toronto, Ontario M5G 2C1, Canada.

出版信息

Science. 1997 Feb 14;275(5302):973-7. doi: 10.1126/science.275.5302.973.

DOI:10.1126/science.275.5302.973

PMID:9020079

Abstract

The telomerase ribonucleoprotein catalyzes the addition of new telomeres onto chromosome ends. A gene encoding a mammalian telomerase homolog called TP1 (telomerase-associated protein 1) was identified and cloned. TP1 exhibited extensive amino acid similarity to the Tetrahymena telomerase protein p80 and was shown to interact specifically with mammalian telomerase RNA. Antiserum to TP1 immunoprecipitated telomerase activity from cell extracts, suggesting that TP1 is associated with telomerase in vivo. The identification of TP1 suggests that telomerase-associated proteins are conserved from ciliates to humans.

摘要

端粒酶核糖核蛋白催化在染色体末端添加新的端粒。一个编码名为TP1（端粒酶相关蛋白1）的哺乳动物端粒酶同源物的基因被鉴定并克隆。TP1与嗜热四膜虫端粒酶蛋白p80表现出广泛的氨基酸相似性，并被证明能与哺乳动物端粒酶RNA特异性相互作用。针对TP1的抗血清从细胞提取物中免疫沉淀端粒酶活性，这表明TP1在体内与端粒酶相关。TP1的鉴定表明，端粒酶相关蛋白从纤毛虫到人类都是保守的。