基于分子的诊断技术在应对作物生物安全和植物检疫问题方面的进展。
Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues.
作者信息
Schaad Norman W, Frederick Reid D, Shaw Joe, Schneider William L, Hickson Robert, Petrillo Michael D, Luster Douglas G
机构信息
USDA/ARS, Foreign Disease-Weed Science Research Unit, Ft. Detrick, Maryland 21702, USA.
出版信息
Annu Rev Phytopathol. 2003;41:305-24. doi: 10.1146/annurev.phyto.41.052002.095435.
Awareness of crop biosecurity and phytosanitation has been heightened since 9/11 and the unresolved anthrax releases in October 2001. Crops are highly vulnerable to accidental or deliberate introductions of crop pathogens from outside U.S. borders. Strategic thinking about protection against deliberate or accidental release of a plant pathogen is an urgent priority. Rapid detection will be the key to success. This review summarizes recent progress in the development of rapid real-time PCR protocols and evaluates their effectiveness in a proposed nationwide network of diagnostic laboratories that will facilitate rapid diagnostics and improved communication.
自2001年9月11日事件以及同年10月未解决的炭疽菌泄露事件以来，人们对作物生物安全和植物检疫的意识有所提高。作物极易受到来自美国境外的作物病原体意外或蓄意引入的影响。针对防范植物病原体的蓄意或意外释放进行战略思考是当务之急。快速检测将是成功的关键。本综述总结了快速实时PCR检测方法开发的最新进展，并评估了其在拟议的全国性诊断实验室网络中的有效性，该网络将促进快速诊断并改善沟通。
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