Stenmark Pål, Nordlund Pär
Department of Biochemistry and Biophysics, Stockholm University, Roslagstullsbacken 15, Albanova University Center, SE-10691 Stockholm, Sweden.
FEBS Lett. 2003 Sep 25;552(2-3):189-92. doi: 10.1016/s0014-5793(03)00920-7.
The alternative oxidase (AOX) is a terminal oxidase present in the respiratory chain of all plants as well as some yeasts and trypanosomes, but has not previously been found in a prokaryote. We have identified an AOX homologue in Novosphingobium aromaticivorans, the first AOX found in a prokaryote. We have cloned the gene for the N. aromaticivorans AOX and showed it to have a terminal oxidase activity when heterologously expressed in Escherichia coli. We have also shown that this novel AOX is expressed in N. aromaticivorans cells, and that its expression level is greatly influenced by the oxygen level and carbon source of the growth media.
交替氧化酶（AOX）是一种末端氧化酶，存在于所有植物以及一些酵母和锥虫的呼吸链中，但此前尚未在原核生物中发现。我们在食芳烃新鞘氨醇菌中鉴定出一种AOX同源物，这是在原核生物中发现的首个AOX。我们克隆了食芳烃新鞘氨醇菌AOX的基因，并证明其在大肠杆菌中异源表达时具有末端氧化酶活性。我们还表明，这种新型AOX在食芳烃新鞘氨醇菌细胞中表达，其表达水平受生长培养基的氧水平和碳源的极大影响。
