硼酸可逆地抑制前体信使核糖核酸剪接的第二步。

Boric acid reversibly inhibits the second step of pre-mRNA splicing.

作者信息

Shomron Noam, Ast Gil

机构信息

Department of Human Genetics, Sackler School of Medicine, Tel Aviv University, 69978 Tel Aviv, Ramat Aviv, Israel.

出版信息

FEBS Lett. 2003 Sep 25;552(2-3):219-24. doi: 10.1016/s0014-5793(03)00928-1.

DOI:10.1016/s0014-5793(03)00928-1

PMID:14527690

Abstract

Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3' splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.

摘要

已有多种方法用于鉴定参与mRNA剪接的因素。然而，这些方法中没有一种包含使用除螯合剂以外的外部试剂来抑制剪接第二步的直接可逆方法。本研究表明，在体外前体mRNA剪接反应中添加硼酸会对剪接的第二步产生剂量依赖性的可逆抑制作用。其作用机制不涉及几种金属离子的螯合、3'剪接位点的阻碍或与hSlu7的结合。本研究提出了一种特异性可逆抑制前体mRNA剪接第二步的新方法。