Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Wiley Interdiscip Rev RNA. 2012 May-Jun;3(3):331-50. doi: 10.1002/wrna.112. Epub 2011 Oct 19.
The molecular mechanisms of the second step of pre-mRNA splicing in yeast and higher eukaryotes are reviewed. The important elements in the pre-mRNA, the participating proteins, and the proposed secondary structures and roles of the snRNAs are described. The sequence of events in the second step is presented, focusing on the actions of the proteins in setting up and facilitating the second reaction. Mechanisms for avoiding errors in splicing are discussed.
本文综述了酵母和高等真核生物前体 mRNA 剪接第二步的分子机制。描述了前体 mRNA 中的重要元件、参与的蛋白质以及 snRNA 的推测二级结构和作用。呈现了第二步中的事件顺序，重点介绍了蛋白质在建立和促进第二反应中的作用。还讨论了避免剪接错误的机制。
