Rosa-Calatrava Manuel, Puvion-Dutilleul Francine, Lutz Pierre, Dreyer Dominique, de Thé Hugues, Chatton Bruno, Kedinger Claude
Transgene S.A., Rue de Molsheim, 67000 Strasbourg, France.
EMBO Rep. 2003 Oct;4(10):969-75. doi: 10.1038/sj.embor.embor943.
The product of adenovirus type 5 (Ad5) gene IX, protein IX (pIX), is a multifunctional protein that stabilizes the viral capsid and has transcriptional activity. We show that pIX also contributes to the Ad5-induced reorganization of the host-cell nuclear ultrastructure: pIX induces the formation of specific and dynamic nuclear inclusions, and the host promyelocytic leukaemia (PML) protein, which is the main structural organizer of PML bodies, is stably relocated and confined within the pIX-induced inclusions late in infection. Our results suggest that Ad5 has evolved a unique strategy that leads to the sustained neutralization of PML bodies throughout infection, thereby ensuring optimal viral proliferation.
5型腺病毒（Ad5）的基因IX产物，即IX蛋白（pIX），是一种多功能蛋白，可稳定病毒衣壳并具有转录活性。我们发现pIX还参与了Ad5诱导的宿主细胞核超微结构重组：pIX诱导形成特定的动态核内包涵体，并且宿主早幼粒细胞白血病（PML）蛋白（PML小体的主要结构组织者）在感染后期稳定地重新定位并局限于pIX诱导的包涵体内。我们的结果表明，Ad5已经进化出一种独特的策略，可在整个感染过程中持续中和PML小体，从而确保最佳的病毒增殖。
